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comstat matlab package  (MathWorks Inc)


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    MathWorks Inc comstat matlab package
    FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by <t>COMSTAT</t> of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.
    Comstat Matlab Package, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/comstat matlab package/product/MathWorks Inc
    Average 90 stars, based on 1 article reviews
    comstat matlab package - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "FleQ finetunes the expression of a subset of BrlR-activated genes to enable antibiotic tolerance by Pseudomonas aeruginosa biofilms"

    Article Title: FleQ finetunes the expression of a subset of BrlR-activated genes to enable antibiotic tolerance by Pseudomonas aeruginosa biofilms

    Journal: Journal of Bacteriology

    doi: 10.1128/jb.00503-24

    FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by COMSTAT of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.
    Figure Legend Snippet: FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by COMSTAT of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.

    Techniques Used: Mutagenesis, Expressing



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    FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by <t>COMSTAT</t> of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.
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    Image Search Results


    FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by COMSTAT of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.

    Journal: Journal of Bacteriology

    Article Title: FleQ finetunes the expression of a subset of BrlR-activated genes to enable antibiotic tolerance by Pseudomonas aeruginosa biofilms

    doi: 10.1128/jb.00503-24

    Figure Lengend Snippet: FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by COMSTAT of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.

    Article Snippet: Quantitative analysis of the images was performed using COMSTAT MATLAB package and COMSTAT2 ( , ).

    Techniques: Mutagenesis, Expressing